Optics and Imaging Centre

About Us

We serve four main areas of activity in microscopy viz.,

  • development and application of specific ultra-structural protocols as required by research-based needs/users. This includes standard electron microscopy procedures as well as specialised techniques such as immunogold labelling, cell culture ultrastructure, ultra-structural image analysis
  • Tissue fixation, automated processing, embedding and microtomy for routine light microscopy stains;
  • Immunohistochemistry protocols inclusive of immunofluorescent staining protocols specific to user demands;
  • Image acquisition, image analysis and data processing;
  • Teaching and training activities to users, undergraduate and postgraduate students.

Advanced research equipment housed at the OIC include:

  • Transmission Electron Microscope -Jeol 1011
  • White Laser TCS SP5X Confocal Laser Scanning Microscope- Leica
  • Axiovision Fluorescent/Brightfield Microscope– Zeiss Axioscope A1 interfaced with Axiovision image analysis software version 4.8.3.
  • Autostainer Link 48 with pre-treatment link –Dako
  • Bioplex Magpix Multiplex Reader with Bioplex manager software version 4.1.1- Biorad

Instrumentation specific for the ancillary preparative techniques allied to each of the above equipment are available at the OIC.  These include:

  • Automated tissue processor -Leica
  • Automated tissue embedder-Leica
  • Automated electron microscopy tissue processor – Lynx
  • Microtome (x2)-Leica and Sakura
  • Ultramicrotomes (x2)-Leica